05045451 is referenced by 68 patents and cites 4 patents.

The present disclosure described an assay for screening monoclonal antibodies for their potential as highly cytotoxic immunotoxins. The assay involves treating cells with dilutions of the test antibody followed by a Fab fragment of a secondary antibody coupled to an A chain toxin ("indirect assay"). The cytotoxicity of the indirect assay is compared to that of the direct assay where the monoclonal antibody is coupled to an A chain toxin. Indirect and direct assays were carried out using 14 antibodies and a panel of 8 human and mouse cell types. The two assays showed virtually 100% correlation. The indirect assay, therefore, predicts the potency of a given monoclonal antibody to make an effective immunotoxin and should be useful in screening monoclonal antibodies for use as immunotoxins.

Title
Methods for screening antibodies for use as immunotoxins
Application Number
7/262974
Publication Number
5045451
Application Date
October 26, 1988
Publication Date
September 3, 1991
Inventor
Ellen S Vitetta
6914 Pemberton Dr., Dallas, 75230
TX, US
Jonathan W Uhr
12355 Montego Plz., Dallas, 75230
TX, US
Assignee
Board of Regents
TX, US
IPC
G01N 33/577
G01N 33/567
G01N 33/563
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