05038852 is referenced by 223 patents and cites 28 patents.

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

Title
Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
Application Number
833368
Publication Number
5038852
Application Date
March 14, 1990
Publication Date
August 13, 1991
Inventor
Joseph T Widunas
Berkeley
CA, US
Larry J Johnson
San Jose
CA, US
Agent
Kevin R Kaster
Ronald C Fish
Assignee
Cetus Corporation
CA, US
IPC
G01N 35/02
F23N 5/20
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