05003059 is referenced by 78 patents and cites 4 patents.

This invention relates to the methods, apparatus, reagents and mixtures of reagents for sequencing natural or recombinant DNA and other polynucleotides. In particular, this invention relates to a method for sequencing polynucleotides based on mass spectrometry to determine which of the four bases (adenine, guanine, cytosine or thymine) is a component of the terminal nucleotide. In particular, the present invention relates to identifying the individual nucleotides by the mass of stable nuclide markers contained within either the dideoxynucleotides, the DNA primer, or the deoxynucleotide added to the primer. This invention is particularly useful in identifying specific DNA sequences in very small quantities in biological products produced by fermentation or other genetic engineering techniques. The invention is therefore useful in evaluating safety and other health concerns related to the presence of DNA in products resulting from genetic engineering techniques.

Title
Determining DNA sequences by mass spectrometry
Application Number
7/209247
Publication Number
5003059
Application Date
June 20, 1988
Publication Date
March 26, 1991
Inventor
Thomas M Brennan
Cupertino
CA, US
Agent
Allegretti & Witcoff
Assignee
Genomyx
CA, US
IPC
C07H 21/04
C07H 21/00
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