04965188 is referenced by 1379 patents and cites 11 patents.

A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

Title
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
Application Number
716975
Publication Number
4965188
Application Date
June 17, 1987
Publication Date
October 23, 1990
Inventor
Randall K Saiki
Richmond
CA, US
Glenn Horn
Emeryville
CA, US
David H Gelfand
Oakland
CA, US
Henry A Erlich
Oakland
CA, US
Kary B Mullis
La Jolla
CA, US
Agent
Albert P Halloin
Janet E Hasak
Kevin R Kaster
Assignee
Cetus Corporation
CA, US
IPC
C12N 15/00
C12P 19/34
C12P 21/00
C12Q 1/68
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