04963489 is referenced by 244 patents and cites 33 patents.

The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts, grown to subconfluence on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.

Title
Three-dimensional cell and tissue culture system
Application Number
853569
Publication Number
4963489
Application Date
September 8, 1988
Publication Date
October 16, 1990
Inventor
Brian A Naughton
Groton
VT, US
Gail K Naughton
Groton
VT, US
Agent
Pennie & Edmonds
Assignee
Marrow Tech
CA, US
IPC
A01N 1/02
C12N 5/00
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