04889818 is referenced by 426 patents and cites 3 patents.

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.

Title
Purified thermostable enzyme
Application Number
899241
Publication Number
4889818
Application Date
June 17, 1987
Publication Date
December 26, 1989
Inventor
Randall K Saiki
Richmond
CA, US
Frances C Lawyer
Oakland
CA, US
Susanne Stoffel
El Cerrito
CA, US
David H Gelfand
Oakland
CA, US
Agent
Albert P Halluin
Kevin R Kaster
Janet E Hasak
Assignee
Cetus Corporation
CA, US
IPC
C12N 9/00
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