04803154 is referenced by 36 patents and cites 6 patents.

An assay for an immunologically active substance (B) in a biologically derived test sample comprising using an immuno-active membrane of a hydrophobic polymer sheet having numerous hydrophilic spots, on which a prefixed amount of an immuno-active substance (A) or (B) has been immobilized, and a coloring-membrane having numerous spots which contain a color-producing reagent being capable of developing color by the catalytic effect of an enzyme at the corresponding positions to those of the immuno-active membrane, and making contact the immuno-active membrane with said test sample in the presence of an enzyme-labeled immuno-active substance (A') or (B') and a substrate for reacting the substance (A), substance (B) and enzyme-labeled substance (A') or (B') competitively or non-competitively, and superposing the coloring-membrane of on the immuno-active membrane, followed by colorimetrically measuring the resulting color and thereby determining the amount of the substance (B) in the test sample.

Title
Multi-spot enzyme immunoassay method
Application Number
6/856018
Publication Number
4803154
Application Date
April 25, 1986
Publication Date
February 7, 1989
Inventor
Shigeru Kaku
Kyoto
JP
Setsuko Nakanishi
Kyoto
JP
Kazuo Horiguchi
Kyoto
JP
Masaji Nishimura
Kyoto
JP
Junko Uo
Kyoto
JP
Agent
Wenderoth Lind & Ponack
Assignee
Shionogi &
JP
IPC
G01N 33/53
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