A test strip is disclosed which provides for transport and separation of heterogenous fluid samples. This test strip can be used for analysis of whole blood by reflectance measurement. This test strip, which can be especially adapted for use in a disposable blood sampling fixture, comprises two functional components: (1) a wicking element for reception of the whole blood sample and transport of the sample to a dry chemistry reagent system, and (2) a porous membrane which has been impregnated with a dry chemistry reagent system specific for analysis of an analyte within the whole blood sample. A barrier layer is provided between the wicking layer and the porous membrane to preclude contact therebetween. In the preferred embodiments of this invention, an aperture is cut or formed in the barrier layer to allow for the flow of sample for the wick to the surface of the porous membrane. Such transport of sample does not, however, involve or contemplate physical between the wick and the membrane in the area of the aperture. This test strip is suitable for use in a disposable fixture designed for self collection and testing of the whole blood sample for one or more constituents (i.e. glucose).