A method and apparatus is provided for measuring the timing jitter of a tributary data stream that has been multiplexed into a higher-rate multiplex stream using pulse stuffing techniques. The required jitter measurement is carried out by monitoring the relevant stuffing control bits of the multiplex stream. In one embodiment, the apparatus comprises a first microprocessor for determining the tributary stuffing ratio from the stuffing control bits, and a second microprocessor operative to determine a current jitter signal value by using both the stuff information contained in the stuffing control bits, and the stuffing ratio determined by the first microprocessor. The second microprocessor carried out its task by updating a preceding jitter signal value by incrementing this value each time a stuff occurs while decreasing the jitter signal value at a rate dependent on the stuffing ratio. Preferred algorithms are disclosed for determining both the stuffing ratio and current jitter signal value.