04725677 is referenced by 312 patents and cites 13 patents.

The invention relates to a process for the preparation of oligonucleotides by the following steps: reaction of a nucleoside with a phosphine derivative, reaction of the nucleotide derivative thus obtained with a nucleoside bonded to a polymeric carrier, oxidation of the carrier-bound nucleoside-nucleotide thus obtained with formation of phosphotriester groups, blocking of free primary 5'-OH groups, elimination of a protective group from the terminal 5'-OH group, where appropriate single or multiple repetition of the abovementioned steps to introduce further nucleoside phosphate or oligonucleoside phosphate units, and cleavage of the nucleoside-carrier bond and, where appropriate, elimination of all protective groups present in the oligonucleoside phosphates. The phosphine derivative used is a compound of the general formula III ##STR1## in which X and L can react with OH groups of the sugar units in the oligonucleotides, and R.sup.3 is a protective group which can be liberated by .beta.-elimination.

Title
Process for the preparation of oligonucleotides
Application Number
6/752178
Publication Number
4725677
Application Date
June 18, 1985
Publication Date
February 16, 1988
Inventor
Nanda D Sinha
Bundu Dt. Ranchi
IN
Hubert Koster
Hamburg
DE
Agent
Hamilton Brook Smith and Reynolds
Assignee
Biosyntech
DE
IPC
C07H 17/00
C07H 15/12
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