04683202 is referenced by 4203 patents.

The present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

Title
Process for amplifying nucleic acid sequences
Application Number
716975
Publication Number
4683202
Application Date
October 25, 1985
Publication Date
July 28, 1987
Inventor
Kary B Mullis
Kensington
CA, US
Agent
Albert P Halluin
Janet E Hasak
Assignee
Cetus Corporation
CA, US
IPC
C07H 21/02
C07H 21/04
C12N 1/00
C12N 15/00
C12P 19/34
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