04683195 is referenced by 3507 patents and cites 1 patents.

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.

Title
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
Application Number
716975
Publication Number
4683195
Application Date
February 7, 1986
Publication Date
July 28, 1987
Inventor
Stephen J Scharf
Berkeley
CA, US
Randall K Saiki
Richmond
CA, US
Glenn T Horn
Emeryville
CA, US
Norman Arnheim
Woodland Hills
CA, US
Henry A Erlich
Oakland
CA, US
Kary B Mullis
Kensington
CA, US
Agent
Albert P Halluin
Janet E Hasak
Assignee
Cetus Corporation
CA, US
IPC
C07H 21/04
C07H 21/02
G01N 33/564
G01N 33/566
G01N 33/00
G01N 33/48
C12N 15/00
C12N 1/00
C12P 19/34
C12Q 1/68
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