04683194 is referenced by 290 patents and cites 4 patents.

In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. The nucleic acid is hybridized to the probe and a blocking oligomer may be added, if necessary, to prevent non-specific binding of the probe. Subsequent digestion with a restriction enzyme cleaves those oligomers that have hybridized to the nucleic acid and reformed the restriction site. The resulting cut and uncut labeled oligomers are separated and detected based on the type of probe label.

The described method may be used to detect sickle cell anemia.

Title
Method for detection of polymorphic restriction sites and nucleic acid sequences
Application Number
614957
Publication Number
4683194
Application Date
March 28, 1985
Publication Date
July 28, 1987
Inventor
Henry A Erlich
Oakland
CA, US
Randall K Saiki
Richmond
CA, US
Agent
Albert P Halluin
Janet E Hasak
Assignee
Cetus Corporation
CA, US
IPC
C12P 19/34
C12N 15/00
C12Q 1/68
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