04494385 is referenced by 48 patents and cites 10 patents.

A method of preserving an organ which comprises a first perfusing step of injecting blood uniform i.e., perfusing choline from an artery or portal vein of the excised organ while gradually lowering its temperature and exhausting it from the vein, continuing until the liquid is lowered to the first proximity of a low temperature before its solidifying temperature, second perfusing step of perfusing refrigerating defect-preventing dimethyl sulfoxide or glycerin agent instead of the blood perfusing liquid while gradually lowering the temperature from the first proximity of a low temperature continuing until the agent becomes the second proximity lowering temperature before its solidifying temperature, third perfusing step of perfusing the final perfusing liquid of low solidifying temperature lower than alcohol or ether instead of the agent while gradually lowering the temperature of the liquid from the second proximity of a low temperature and continuing until the liquid reaches the third proximity of a low temperature before its solidfying temperature, or until the liquid is frozen.

Title
Method of preserving organ and apparatus for preserving the same
Application Number
499220
Publication Number
4494385
Application Date
May 20, 1984
Publication Date
January 22, 1985
Inventor
Nobuo Sakao
Sapporo
JP
Yasuo Kuraoka
Sapporo
JP
Agent
George B Oujevolk
Assignee
Hoxan Corporation
JP
IPC
B01F 3/04
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