04418148 is referenced by 378 patents and cites 6 patents.

A contiguous multilayer membrane for use with an electrochemical sensor is prepared comprising a first relatively nonporous dense polymer layer, a second polymer layer less dense and more porous than the first layer, a third layer containing an enzyme and a fourth polymer layer less dense and more porous than the first layer. The polymer is preferably cellulose acetate and the second and fourth layers are prepared with a solvent and nonsolvent for the polymer. The membrane may contain multiple enzyme layers separated by a polymer layer. The multilayer membrane provides advantages of higher substrate conversion, homogeneous distribution of enzyme and/or minimized interference with analyte diffusion.

Title
Multilayer enzyme electrode membrane
Application Number
6/318627
Publication Number
4418148
Application Date
November 5, 1981
Publication Date
November 29, 1983
Inventor
Bruce J Oberhardt
Mishawaka
IN, US
Agent
Roger N Coe
Assignee
Miles Laboratories
IN, US
IPC
C12M 1/40
C12N 11/04
C12N 11/08
C12N 11/12
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