04299916 is referenced by 216 patents and cites 8 patents.

An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) of immunogens--ligand and receptor. The method has two basic elements: a solid surface to which one of the members of the immunological pair is bonded and a signal producing system, which includes a catalytic member bonded to a mip, which signal producing system results in a measurable signal on said solid surface related to the amount of analyte in the medium. The signal generating compound is produced without separation of the catalyst labeled mip bound to the solid surface from the catalyst labeled mip free in solution.

In a preferred embodiment, an enzyme is bonded to a mip which acts in conjunction with a solute to produce a signal generating product which binds preferentially to the solid surface when the enzyme is bound to the surface, resulting in a signal which is readily differentiated from signal generating compound produced by the catalyst and solute in the bulk solution.

Title
Preferential signal production on a surface in immunoassays
Application Number
6/106620
Publication Number
4299916
Application Date
December 26, 1979
Publication Date
November 10, 1981
Inventor
Edwin F Ullman
Atherton
CA, US
David J Litman
Palo Alto
CA, US
Agent
Bertram I Rowland
Assignee
Syva Company
CA, US
IPC
G01N 1/48
C12Q 1/68
G01N 33/54
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