04228237 is referenced by 117 patents and cites 4 patents.

A method for determining the presence of a ligand in a liquid medium which utilizes enzyme labeled avidin and biotin labeled reagent in a specific binding process wherein the ligand to be detected is contacted with an insoluble phase containing specific binding substance for the ligand. Various protocols may be used to assay the resulting enzyme activity which is related to the amount of ligand in the liquid medium.

Title
Methods for the detection and determination of ligands
Application Number
5/944254
Publication Number
4228237
Application Date
September 21, 1978
Publication Date
October 14, 1980
Inventor
Mark K Malmros
Newton
PA, US
Richard C Hevey
Rockport
ME, US
Agent
Natalie Jensen
Assignee
Calbiochem Behring
CA, US
IPC
C12Q 1/66
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