04098876 is referenced by 355 patents and cites 4 patents.

Method of determining the presence and/or concentration of a polyvalent antigenic substance in a fluid. The method comprises the steps of incubating the fluid with labeled antibodies to the substance to form a first labeled immunochemical complex and then incubating that complex with immobilized antibodies to the substance to form a second labeled complex which is separated from the incubation medium. The amount of label in the second complex provides a means for detecting and/or quantitating the substance in the fluid. By reversing the sequence of incubation steps in a "two-site" or sandwich assay, greater sensitivity is achieved and an intermediate washing step is eliminated.

Title
Reverse sandwich immunoassay
Application Number
5/735740
Publication Number
4098876
Application Date
October 26, 1976
Publication Date
July 4, 1978
Inventor
James E Woiszwillo
Milford
MA, US
James W Ryan
Miami
FL, US
Roger N Piasio
Medfield
MA, US
Agent
Clarence R Patty Jr
Clinton S Janes Jr
William E Maycock
Assignee
Corning Glass Works
NY, US
IPC
A61K 43/00
G01N 33/16
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