The present invention relates to a process for preparing anthrax protective antigen protein from E. coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and high-level production of anthrax PA from E. coli. The steps of the process involves, transforming E. coli DH5α cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5α cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

Title
High level constitutive production of anthrax protective antigen
Application Number
10/494680
Publication Number
20050054038
Application Date
December 7, 2001
Publication Date
March 10, 2005
Inventor
Vibha Chauhan
Waheed Sayed Mohsin
Rakesh Bhatnagar
Agent
Ladas & Parry
NY, US
IPC
C07K 17/00
C07K 16/00
C12P 21/06
C07K 14/00
C07K 01/00
A23J 01/00
A61K 39/07
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