A vector comprising one recognition sequence of a first restriction enzyme of which digestion site exists at a particular position with respect to the recognition sequence and does not exist within the recognition sequence and one recognition sequence of a second restriction enzyme of which digestion site is specific, wherein a fragment obtained by digestion of the vector with the first restriction enzyme can be digested with the second restriction enzyme and a distance between the digestion site of the first restriction enzyme and the digestion site of the second restriction enzyme is 30 nucleotides or shorter, and a method for producing DNA having a target sequence by using the vector.

Title
Method for producing DNA
Application Number
10/298491
Publication Number
20030109009
Application Date
November 15, 2002
Publication Date
June 12, 2003
Inventor
Satoshi Aotsuka
Agent
Knobbe Martens Olson & Bear
CA, US
IPC
C12N 15/85
C12N 15/00
C12P 19/34
View Original Source