The systems and methods described herein relate to nucleic acid probes comprising a pattern of universal and designate nucleotides (or analogs thereof), or ‘gapped’ probes, and the use of sets of gapped probes in sequencing by hybridization to determine the sequence of nucleic acid sequences. The inclusion of universal nucleotides in the probes allows for efficient and rapid sequencing of longer nucleotide sequences than can be sequenced using traditional probes. The systems and methods described herein also relate to apparatus for sequencing nucleic acids which include gapped probes, as well as computer systems capable of analyzing data generated using gapped probes in such apparatus.

Title
Systems and methods for sequencing by hybridization
Application Number
10/095363
Publication Number
20030064382
Application Date
March 7, 2002
Publication Date
April 3, 2003
Inventor
John S Oliver
Bristol
RI, US
Eliezer Upfal
Providence
RI, US
Franco P Preparata
Providence
RI, US
Agent
Ropes & Gray
MA, US
IPC
C07H 21/04
C12Q 01/68
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