Methods and compositions are described for the efficient delivery and expression of recombinant polynucleotides in animal cells in vitro or in vivo. In particular, a comprehensive approach of non-viral gene delivery is provided that has been optimized with regards to the vectors delivered, maximizing the amount and duration of gene expression, and methods of conditioning the patient to enhance the efficiency of gene delivery. The described methods find application in both gene therapy, and the functional analysis of cloned gene products in vivo.

Title
Methods and compositions for nonviral gene delivery
Application Number
9/232175
Publication Number
20020065213
Application Date
January 15, 1999
Publication Date
May 30, 2002
Inventor
Robert J Debs
MILL VALLEY
CA, US
Agent
Tracy J Dunn IP
CA, US
IPC
C12N 15/87
C12N 05/08
C12N 05/06
C12P 21/02
A61K 38/17
A61K 48/00
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