A method for monitoring the microbiological contamination of opaque media is described and claimed. In this method, a Fluorogenic Dye is added to an Aliquot of opaque medium. After a certain time period, a fluorometer capable of measuring fluorescent signals in an opaque medium is used to measure the fluorogenic signals of the Fluorogenic Dye and the Reacted Fluorogenic Dye. A Useful RATIO of the fluorescent signal of the Reacted Fluorogenic Dye to the fluorescent signal of the Fluorogenic Dye is calculated and the information gleaned from the RATIO is used to ascertain the state of microbiological contamination in the opaque medium.

Title
Measurement of microbiological activity in an opaque medium
Application Number
9/894929
Publication Number
20020042092
Application Date
June 28, 2001
Publication Date
April 11, 2002
Inventor
David P Workman
Naperville
IL, US
Sasireka S Ramesh
Aurora
IL, US
Michael J Fehr
Geneva
IL, US
Ronald V Davis
Geneva
IL, US
Mita Chattoraj
Warrenville
IL, US
Nancy L Casselman
Aurora
IL, US
Rodney H Banks
Aurora
IL, US
Agent
ONDEO Nalco Company
IL, US
Patent & Licensing Department
IL, US
IPC
C12Q 01/06
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