PROBLEM TO BE SOLVED: To provide an automatic sample testing apparatus to test a sample housed in a test card. SOLUTION: This apparatus 20 has a test sample array system 100 to move a tray 22 for housing a plurality of test sample cards 28 and a fluid container between various stations therein. The machine 20 has a vacuum station 400 having a vacuum chamber movable freely above or below the tray and a fluid sample is loaded into a card from the container when vacuum is released. The test sample array system moves the tray to a cutting and sealing station 500 and then to an incubation station 600. The test cards are further transferred to an optically reading station 800 and a well of the cards undergo optical measurement. After reading, the cards are returned to the station 600 for the subsequent incubation and reading or transferred to a stacking card disposing system to complete the reading.