1138784-A2 is referenced by 77 patents and cites 3 patents.

The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.

Title
Method for determining the efficiency of nucleic acid amplifications
Application Number
EP20010107738 20010402
Publication Number
1138784 (A2)
Application Date
April 2, 2001
Publication Date
October 4, 2001
Inventor
Gutekunst Martin Dr
DE
Soong Richie Dr
DE
Sagner Gregor Dr
DE
Tabiti Karim Dr
DE
Assignee
Roche Diagnostics
DE
IPC
G06F 19/00
C12Q 01/68
C12Q 01/68
C12Q 01/68
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