0731163-A2 is referenced by 5 patents and cites 4 patents.

A process for preparing a culture skin which comprises the following steps: a) a step in which a culture skin matrix comprising a collagen sponge, a collagen sheet or a collagen gel is prepared in a means having a projection to provide said matrix with a penetrating pore, b) a step in which a skin-derived cell is seeded and cultured on said matrix, and c) a step in which the culture skin is provided with a penetrating pore by the means having the projection before said culture skin covers a skin defect, if necessary, the steps a) and b) are simultaneously carried out; a culture skin matrix and a culture skin obtained thereby.; According to the present invention, a culture skin can be prepared wherein sufficient nutrition supply to epidermal cells and/or fibroblasts cultured and proliferated and discharge of exudate excessively pooled in the wound can be smoothly performed, while contraction is inhibited, by producing a penetrating pore in the culture skin before covering the wound with the culture skin with a means which requires no pretreatment in order to prevent the flowing out and losing of cells which are caused in seeding cells on a collagen matrix.

Title
Culture skin and process for preparing the same
Application Number
EP19960103395 19960305
Publication Number
0731163 (A2)
Application Date
March 5, 1996
Publication Date
September 11, 1996
Inventor
Hamano Kyoko
JP
Moriyama Takeshi
JP
Sugiyama Akihisa
JP
Assignee
Menicon
JP
IPC
C12N 05/08
C12N 05/08
C12N 05/06
C12N 05/00
A61L 27/00
C12N 05/08
C12N 05/06
C12N 05/00
A61L 27/00
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