0488769-A2 is referenced by 92 patents and cites 21 patents.

An instrument for performing highly accurate PCR employing a sample block in microtiter tray format. The sample block has local balance and local symmetry. A three zone film heater controlled by a computer and ramp cooling solenoid valves also controlled by the computer for gating coolant flow through the block controls the block temperature. Constant bias cooling is used for small changes. Sample temperature is calculated instead of measured. A heated cover deforms plastic caps to apply a minimum acceptable threshold force for seating the tubes and thermally isolates them. The control software includes diagnostics. An install program tests and characterizes the instrument. A new user interface is used. Disposable, multipiece plastic microtiter trays to give individual freedom to sample tubes are taught.

Title
Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control.
Application Number
EP19910311090 19911129
Publication Number
0488769 (A2)
Application Date
November 29, 1991
Publication Date
June 3, 1992
Inventor
Leath Richard
US
Ragusa Robert P
US
Margulies Marcel
US
Woudenberg Timothy M
US
Williams Fenton
US
Atwood John Girdner
US
Goven Lisa May
US
Mossa Albert Carmelo
US
Assignee
Perkin Elmer Cetus Instr
US
IPC
C12Q 01/68
B01L 09/06
B01J 19/00
C12P 19/00
G05D 23/19
G05B 13/00
G01N 35/00
C12Q 01/24
C12Q 01/00
C12P 19/34
C12N 15/09
C12N 15/00
C12M 01/40
C12M 01/38
C12M 01/36
B01L 09/00
B01L 07/00
B01L 03/14
B01L 03/00
C12M 01/00
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