0381501-A2 is referenced by 95 patents and cites 6 patents.

There are disclosed a cuvette (10, 100, 100C, 100D, 100E) and a method of use which prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments (30, 30A, 30B, 32, 32A, 32B, 34, 34A, 34B, 36, 36A, 36B, 38, 38A, 38B, 40, 40A, 40B) and (132, 132A, 132B, 132C, 132D, 132E, 134, 134A, 134B, 134C, 134D, 134E, 136, 136A, 136B, 136C, 136D, 136E, 138, 138A, 138B, 138C, 138D, 138E, 139, 139A, 139B, 139C, 139D, 139E) in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released.; Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source (60), or via pistons (113, 113C, 113D, 115, 115C, 115D, 184, 184C, 184E, 260) that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

Title
Containment cuvette for pcr and method of use.
Application Number
EP19900301061 19900201
Publication Number
0381501 (A2)
Application Date
February 1, 1990
Publication Date
August 8, 1990
Inventor
Findlay John Bruce C O Eastman
Donish William Harold C O East
Hinckley Charles Cullis C O Ea
Wellman Jeffrey Allen C O East
Seaberg Leonard Joseph C O Eas
Schnipelsky Paul Nicholas C O
Assignee
Eastman Kodak Co
US
IPC
G01N 21/03
C12Q 01/68
B01L 03/00
C12Q 01/68
C12N 15/09
B01L 03/00
C12M 01/00
C12Q 01/68
C12N 15/09
B01L 03/00
C12M 01/00
View Original Source Download PDF