0270822-A1 is referenced by 50 patents and cites 8 patents.

Binary vectors for the introduction of DNA into plant cells are presented. They contain the origin of replication of the Pseudomonas plasmid pVS1 and and a T-DNA region consisting of the border sequences of the TL-DNA of the pTiB6S3 plasmid and a nos-NPTII chimaeric gene to select kanamycin resistance in plant cells. The vectors replicate completely stably in Agrobacterium. Transformation of tobacco protoplasts using the binary vector is equally efficient as for previously described vector systems. The transferred T-DNA is delineated by the border sequences and in general only one or few copies of the T-DNA become integrated into the plant genome.

Title
Stable binary agrobacterium vectors and their use.
Application Number
EP19870115985 19871030
Publication Number
0270822 (A1)
Application Date
October 30, 1987
Publication Date
June 15, 1988
Inventor
Deblaere Rolf Dr
BE
Leemans Jan Dr
BE
Assignee
Max Planck Gesellschaft
DE
IPC
A01H 01/00
C12N 15/00
C12N 15/82
C12N 15/09
A01H 01/00
C12N 15/82
C12N 15/09
A01H 01/00
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