0258017-A2 is referenced by 151 patents and cites 7 patents.

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The amplification process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified.; The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.

Title
Purified thermostable enzyme and process for amplifying, detecting, and/or cloning nucleic acid sequences using said enzyme.
Application Number
EP19870307433 19870821
Publication Number
0258017 (A2)
Application Date
August 21, 1987
Publication Date
March 2, 1988
Inventor
Gelfand David Harrow
Lawyer Frances Cook
Mullis Kary Banks
Stoffel Susanne
Saiki Randall Keichi
Horn Glenn
Erlich Henry Anthony
Assignee
Cetus
US
IPC
C12Q 01/68
C12P 19/34
C12N 15/00
C12N 09/96
C12N 09/12
C12Q 01/68
C12P 19/00
C12N 15/09
C12N 09/96
C12N 09/12
C12Q 01/68
C12P 19/34
C12N 15/09
C12N 09/96
C12N 09/12
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