0200362-A2 is referenced by 162 patents and cites 5 patents.

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired. In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.

Title
Process for amplifying, detecting, and/or cloning nucleic acid sequences.
Application Number
EP19860302298 19860327
Publication Number
0200362 (A2)
Application Date
March 27, 1986
Publication Date
November 5, 1986
Inventor
Scharf Stephen Joel
Horn Glenn Thomas
Erlich Henry Anthony
Saiki Randall Keichi
Arnheim Norman
Mullis Kary Banks
Assignee
Cetus
US
IPC
C12Q 01/68
C12P 19/34
C12N 15/10
C12N 15/00
C07H 21/00
C12Q 01/68
B01L 07/00
C12N 15/09
C12Q 01/68
B01L 07/00
C12N 15/09
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