0130756-A1 is referenced by 186 patents and cites 4 patents.

Methods and vectors are provided for the production of procaryotic carbonyl hydrolases in recombinant systems. DNA which encodes such hydrolases are mutated at predetermined regions by known methods or by a novel point mutagenesis method in order to generate mutant hydrolases. Particular point mutations in carbonyl hydrolases such as subtilisin result in modifications of oxidation stability, Km, Kcat, Kcat/Km ratio substrate specificity, specific activity or pH-activity profiles. These mutated hydrolases are particularly useful in laundry compositions. Mutations in the genes encoding the subtilisin or neutral protease of bacillus yield substantially normally sporulating bacillus strains which are incapable of excreting subtilisin or neutral protease. Such strains are useful in the recombinant synthesis of heterologous proteins.

Title
Procaryotic carbonyl hydrolases, methods, dna, vectors and transformed hosts for producing them, and detergent compositions containing them.
Application Number
EP19840304252 19840622
Publication Number
0130756 (A1)
Application Date
June 22, 1984
Publication Date
January 9, 1985
Inventor
Henner Dennis James
US
Estell David Aaron
US
Wells James Allen
US
Ferrari Eugenio
US
Bott Richard Ray
US
Assignee
Genentech
US
IPC
C12R 01/125
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C12N 15/00
C12N 09/54
C12N 09/14
C11D 07/42
C11D 03/386
C12N 15/74
C12N 15/57
C12N 15/10
C12N 15/09
C12N 15/00
C12N 09/52
C12N 09/50
C12N 09/26
C12N 09/14
C12N 01/21
C12N 01/20
C11D 07/22
C11D 03/38
C07H 21/00
C12N 15/75
C12N 15/57
C12N 15/10
C12N 15/09
C12N 15/00
C12N 09/58
C12N 09/56
C12N 09/54
C12N 09/26
C12N 09/14
C12N 01/21
C12N 01/20
C11D 07/42
C11D 03/386
C07H 21/04
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