0092163-A2 is referenced by 35 patents and cites 2 patents.

Interleukin 2 (IL 2; T-cell growth factor), produced with and without costimulation by Burkitt's lymphoma line Daudi, is purified approximately 37,000-fold to apparent homogeneity from lymphocyte-conditioned medium by (NH4)2SO4- precipitation, ion-exchange chromatography, gel filtration and hydrophobic chromatography. Native IL 2 produced in the absence of Daudi cells has a molecular weight of about 26,000 daltons as measured by gel filtration and yields IL 2 having two molecular weights of about 16,000 and 17,000 daltons after denaturation as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. IL 2 produced in the presence of Daudi cells shows a molecular weight of approximately 14,500 daltons as measured by both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Title
Purification of interleukin 2.
Application Number
EP19830103582 19830414
Publication Number
0092163 (A2)
Application Date
April 14, 1983
Publication Date
October 26, 1983
Inventor
Venuta Salvatore
Welte Karl
Mertelsmann Roland
Assignee
Sloan Kettering Inst Cancer
US
IPC
C07G 07/00
A61K 37/02
C12P 21/00
C07K 14/435
C07K 01/00
A61P 37/00
A61P 35/00
A61K 38/00
C12P 21/00
C07K 14/55
C07K 14/54
C07K 14/52
C07K 14/47
C07K 14/435
C07K 01/20
A61P 37/00
A61P 35/00
A61K 38/00
C12P 21/00
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