The baculovirus-insect cell vector system is used as a high efficiency eukaryotic cloning and expression method for the production of recombinant proteins. A major application of this system is also in the development of rDNA vaccines for a variety of important human and animal diseases. This system is used to express a variety of candidate vaccine immunogens derived from the envelope gene of Human Immunodeficiency Virus (HIV). The envelope protein of HIV is the most promising candidate in the development of vaccines. HIV antigens produced from some of these recombinants have been successfully employed in a variety of immunodiagnostic assays including ELISA, Western Blotting and radioimmunoprecipitation.

Title
Polypeptides derived from envelope gene of human immunodeficiency virus in recombinant baculovirus infected insect cells
Application Number
87107837
Publication Number
1023007
Application Date
October 16, 1987
Publication Date
December 21, 1988
Inventor
Mark A Cochran
Gale E Smith
Franklin Volvovitz
Agent
YANG JIUCHANG
Assignee
Usa
Connecticut 06516
West Haven
Microgenesys
IPC
G01N 33/569
C12P 21/02
C12N 15/00