The invention provides a method for generating autologous retinal stem cells and autologous retinal cells by reversely differentiating human body cells. The method comprises the following steps of: culturing the human body cells in steps in a culture medium which contains different plant extracts and proteins, so that the human body cells are reversely differentiated to generate the autologous retinal stem cells and then continuously generate a plurality of autologous retinal cells. Tens of billions of a first generation of autologous retinal stem cells are generated within 2 to 3 weeks. The plant and protein-induced human autologous retinal stem cells have the same cell specific phenotype as human natural retinal stem cells and various cell differentiation abilities. The autologous retinal stem cells and the autologous retinal cells have good application prospect in the field of treating diseases, such as retinal pigment degeneration, and also have good potential application prospect in the fields of regenerative medicine and autologous tissue organ engineering. The autologous retinal stem cells and the autologous retinal cells have the possibility to be produced massively in an industrialization way.

Title
Method for generating autologous retinal stem cells and autologous retinal cells by reversely differentiating human body cells, kit and application of autologous retinal stem cells and autologous retinal cells
Application Number
201110022102
Publication Number
102604886A
Application Date
January 20, 2011
Publication Date
July 25, 2012
Inventor
Liao Huanzhao
Ren Haiying
Li Guiling
Shi Haipeng
Lin Xiongbin
Assignee
Beijing CSC Creation Biotechnology
IPC
A61P27/02
A61K35/12
C12N5/074
C12N5/071