Methods and compositions to activate a genetically designated target cell (or population of targetcells) artificially, in vivo or in vitro, employ triggering of heterologous stimulus-gated ion channels to activate the cells. The stimulus-gated ion channels are suitably TRPV1, TRPM8 or P2X2. A stimuluswhich leads to opening or 'gating' of the ion channel can be a physical stimulus or a chemical stimulus.Physical stimuli can be provided by heat, or mechanical force, while chemical stimuli can suitably be a ligand, such as capsaicin for TRPV1 or ATP for P2X2, or a 'caged ligand,' for example a photolabileligand derivative, in which case a physical signal in the form of light is used to provide the chemical signal. Selective activation of the cell may be used for various applications including neuronal and neuroendocrine mapping and drug screening.

Title
Heterologous stimulus-gated ion channels and methods of using same
Application Number
03817754
Publication Number
1672055
Application Date
June 2, 2003
Publication Date
September 21, 2005
Inventor
Zemelman Boris V
Miesenbock Gero A
Agent
wang jingchao
Assignee
Sloan Kettering Inst Cancer
IPC
C07K 01/00
C07H 21/04
C12N 01/20
C12P 21/06
G01N 33/567